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Instructions

Use at suggested g/L and supplement with an amino acid mixture (CSM, SC, HSM or BSM) if desired. Mix with stirring for 10-15 minutes and filter sterilize or autoclave at 121°C for 15 minutes. Note: If using an SC amino acid mixture to prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving.

Suggested g/L: 26.71

Storage Temperature: Room Temp

Recipe in mg/L:

SALTS, VITAMINS, MINERALS & TRACE ELEMENTS

Biotin (0.002 mg/L)

Boric acid (0.5 mg/L)

Calcium chloride dihydrate (100 mg/L)

Copper (II) sulfate pentahydrate (0.04 mg/L)

Folic acid (0.002 mg/L)

Inositol (In Base Formula) (2 mg/L)

Iron (III) chloride (0.2 mg/L)

Magnesium sulfate anhydrous (500 mg/L)

Manganese sulfate monohydrate (0.4 mg/L)

Niacin (0.4 mg/L)

4-Aminobenzoic acid (PABA) (In Base Formula) (0.2 mg/L)

D-Pantothenic acid hemicalcium salt (0.4 mg/L)

Potassium iodide (0.1 mg/L)

Potassium phosphate monobasic anhydrous (1000 mg/L)

Pyridoxine hydrochloride (0.4 mg/L)

Riboflavin (0.2 mg/L)

Sodium chloride (100 mg/L)

Sodium molybdate (0.2 mg/L)

Thiamine hydrochloride (0.4 mg/L)

Zinc sulfate monohydrate (0.4 mg/L)

NITROGEN

Ammonium sulfate (5000 mg/L)

SUGAR

Glucose (20000 mg/L)